collagen filaments in the alveolar region of human precision cut lung slices, acquired by 2-photon microscopy
3D render and displacement tracks of AT2 (GFP+) cell adopting a large, flattened, and curved morphology during migration (click on the image to watch video)
3D render and displacement tracks of AT2 (GFP+) cell migrating along the alveolar septum in intact, injured lung tissue (click anywhere on the image to watch video)
AT2 (GFP+) 3dpi moving and showing "protrusion like" structures (click anywhere on the image to watch video)
AT2 (GFP+) moving across the alveolar boundary (click anywhere on the image to watch video)
AT2 (GFP+) cell adopting a large, flattened, and curved morphology during migration (click anywhere on the image to watch video)
3D render and displacement tracks of AT2 (GFP+) cell moving through alveolar pore to the neighboring alveolus
overlapping frames of three distinct AT2s (GFP+) cells phenotypes (cuboidal, elongating and enlarging) following injury. Displacement length is shown as a function of color
human (left) and mouse (right) intact lung tissue slices cultured ex vivo.
3D render and displacement track of a single AT2 (GFP+) cell migrating along the alveolar septum (click anywhere on the image to watch video)
3D render and displacement track of a single AT2 (GFP+) cell moving and adopting an elongated shape within same alveolus (click anywhere on the image to watch video)
evolution of the elongated AT2 behavioral phenotype according to area, displacement, ellipticity and sphericity as a function of color (max values at the final frame showed).
seeing is believing
We are fascinated with dynamical systems in lung injury and regeneration. Changes in chromatin and gene regulation drive changes in cell state and behavior that ultimately drive lung repair or, in some cases, disease. Our lab uses an array of different approaches to investigate these changes, from single cell multi-omics to live in vivo imaging of individual cells in the lung. Our ultimate goal is to uncover mechanisms that govern stem cell fate and function in the lung, and how these are modulated in the context of disease.
NEWS
breaking
NEWS breaking
We’re thrilled to see our work on motility and migration of lung is highlighted in Science magazine!
join us!
Our lab welcomes people of all backgrounds. We are always looking for trainees who are excited about stem cells and regeneration, genetics, intravital microscopy, cell biology, lung biology and disease, stem cell-niche interactions and more!
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confocal image of mouse PCLS. AT2 (GFP), other cells type (tdT) and SiR-DNA (magenta)